acsA [2017-11-03 09:07:28]

acetyl-CoA synthetase

Locus: BSU29680

Isoelectric point: 5.55

Molecular weight: 64.72 kDa

Protein length: 572 aa

Gene length: 1716 bp

Function: utilization of acetate, fatty acids

Product: acetyl-CoA synthetase)

Essential: no

E.C.: 6.2.1.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

Gene

Coordinates: 3,038,213 → 3,039,931

The protein

Catalyzed reaction/ biological activity

• ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• YtcI

Modification

• acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed

Structure

• 2P2F (from Salmonella enterica, 37% identity) PubMed

Expression and Regulation

Operons

• Genes: acsA-ytzK
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by glucose (4.5-fold) (CcpA) PubMed

• Genes: acsA-ytzK-tyrS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by glucose (4.5-fold) (CcpA) PubMed

Biological materials

Mutant

• GP1212 (acsA::kan), available in Stülke lab
• BKE29680 (ΔacsA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCAAACATCCCCCTT, downstream forward: _UP4_ACAATGGAGGATTAAAAGGC
• BKK29680 (ΔacsA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCAAACATCCCCCTT, downstream forward: _UP4_ACAATGGAGGATTAAAAGGC

References

Reviews

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Original publications

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